Usually, DNA from an individual organism is first amplified (mutiple copies made) by the polymerase chain reaction. It is then cut into restriction fragments at defined positions by restriction enzymes. The restriction fragments are then separated by length using agarose gel electrophoresis. The resulting gel may be enhanced by southern bloting. |
Usually, DNA from an individual organism is first amplified (multiple copies made) by the polymerase chain reaction. It is then cut into restriction fragments at defined positions by restriction enzymes. The restriction fragments are then separated by length using agarose gel electrophoresis. The resulting gel may be enhanced by southern bloting. |