Restriction fragment length polymorphism is a property of
DNA used in
molecular biology methods, for example, [genetic fingerprint]
? or [paternity test]
?ing.
Method
Usually, DNA from an individual
organism is first manifolded by
polymerase chain reaction. It is then cut into
restriction fragments at defined positions by
restriction enzymes. The restriction fragments are then separated by length using
agarose gel electrophoresis. The resulting gel may be enhanced by
southern bloting.
Result
The position of certain
gel bands will differ between individuals (thus
polymorphism). This can be used to gentically tell individuals apart. It can also show the genetic relationship between individuals, as a child will inherit genetic elements from its parents. It is also used to deterimne the relationship of
species.
- See also : Genetics -- Retroposon