Sequencing

In genetics, sequencing means to determine the nucleotides of a DNA strand. Methods to do this are :

• chromosome walking
• shotgun sequencing
• Chain termination method

In biochemistry, sequencing means more generally the determination of the primary sequence of any heterobiopolymer. This includes nucleotide sequencing as well as protein sequencing (using Edman degradation, [mass spectrometry]?, protease digests), though it is not common to talk of sequencing a polysaccharide.