* A color marker, e.g. bomide phenole (blue) |
* A color marker, i.e. a low molecular weight dye such as "bromophenol blue", to enable tracking the progress of the electrophoresis. |
# Add 1 ml ethidium bromide on each 10 ml gel solution. Wear double gloves or nitrile gloves from here on, ethidium bromide is a mutagene?! |
# Add 1 ml ethidium bromide on each 10 ml gel solution. Wear double gloves or nitrile gloves from here on, ethidium bromide is a mutagen! |
(1) The agarose gel with three slots (S). (2) Injection of DNA ladder into the first slot. (3) DNA ladder injected. Injection of samples into the second and third slot. (4) A current is applied. The DNA moves toward the positive current. (5) Small DNA strands move fast, large DNA strands move slowly through the gel. (6) The DNA is spread over the whole gel. The electrophoresis process is finished. |
:(1) The agarose gel with three slots (S). :(2) Injection of DNA ladder into the first slot. :(3) DNA ladder injected. Injection of samples into the second and third slot. :(4) A current is applied. The DNA moves toward the positive current. :(5) Small DNA strands move fast, large DNA strands move slowly through the gel. The DNA is not normally visible during this process, so the marker dye is added to the DNA to avoid the DNA being run entirely off the gel. The marker dye has a low molecular weight, and migrates faster than the DNA, so as long as the marker has not run past the end of the gel, the DNA will still be in the gel. :(6) The DNA is spread over the whole gel. The electrophoresis process is finished. |
Put the gel on an UV lamp to view the DNA bands. Wear protective glasses! The DNA can also be cut out of the gel, and the can then be dissolved to retrieve the purified DNA. |
Illuminate the gel with a UV lamp (usually on a light box) to view the DNA bands- ethidium bromide fluoresces pink in the presence of DNA. Wear protective glasses! The DNA can also be cut out of the gel, and can then be dissolved to retrieve the purified DNA. |
/Talk? |
/Talk? |
Illuminate the gel with a UV lamp (usually on a light box) to view the DNA bands- ethidium bromide fluoresces pink in the presence of DNA. Wear protective glasses! The DNA can also be cut out of the gel, and can then be dissolved to retrieve the purified DNA.
/Talk?