Malphigi used the microscope for studies on skin, kidney, and for the first interspecies comparison of the liver. He also described the development of the chick? in its egg?, and discovered that insects (particulary, the [silk worm]?) do not use lung?s to breath, but small holes in their skin called trachea?. A skin layer was named after him, the [Malpighi layer]?, also other structures, like the [Malpighi bodies]? in the liver. Malphigi died on September 29, 1694?, in Rome.