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In [Western blotting]?, proteins are first seperated by size, on an SDS page gel. The proteins on the gel are then tranferred to a PVDF or nitrocellulose membrane. This membrane can then be blotted with solutions of antibodies. Antibodies that bind to the protein of interest can then be visualized by a variety of techniques, including chemoluminescence, or radioactivity. |
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In [Western blotting]?, proteins are first seperated by size, on an SDS page gel. The proteins on the gel are then tranferred to a PVDF or nitrocellulose membrane. This membrane can then be probed with solutions of antibodies. Antibodies that bind to the protein of interest can then be visualized by a variety of techniques, including chemoluminescence or radioactivity. |
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